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opuBD [2017-11-24 14:26:33]
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opuBD [2017-11-24 14:26:33]

choline ABC transporter (membrane protein)
locus
BSU33700
pI
10.06
mw
23.78 kDa
protein length
226 aa Sequence Blast
gene length
678 bp Sequence Blast
function
compatible solute transport
product
choline ABC transporter (membrane protein)
essential
no
synonyms
proZ
Outlinks
KEGGBsubCycSubtiListUniProtExpression Browser

Genomic Context

      

categories

  • [category|SW 4|Lifestyles] → [category|SW 4.3|Coping with stress] → [category|SW 4.3.6|Coping with hyper-osmotic stress]
  • [category|SW 6|Groups of genes] → [category|SW 6.2|Membrane proteins]

    • Gene

    Coordinates
    3,459,806 → 3,460,486

    The protein

    Protein family

  • CysTW subfamily (according to Swiss-Prot)

    • Paralogous protein(s)

  • [protein|76C8D6BF2CA1FA5E7392C52FFD55FD01EF5AB9DC|OpuCD]

    • [SW|Localization]

  • cell membrane [Pubmed|10092453]

    • Expression and Regulation

    Operons

    genes
    [gene|3F502A4DCB1DAD7C3F968465B13C35213240515B|opuBA]-[gene|70B45E7428DB1F6AEF7D2FE3062CC86BE96674FC|opuBB]-[gene|C48798FE13B1521236F5BC5786B9A02D7BC9A336|opuBC]-[gene|1532EC3D741C5AB3D0B642741218FAE00AD460FA|opuBD]
    description
    [Pubmed|10216873]

    sigma factors

  • [protein|360F48D576DE950DF79C1A2677B7A35A8D8CC30C|SigA]: sigma factor, [Pubmed|10216873], in [regulon|360F48D576DE950DF79C1A2677B7A35A8D8CC30C|SigA regulon]

    • regulatory mechanism

  • [protein|5C398C1181CE25CAC079AFBA5871C0B72AE1AA96|GbsR]: repression, (transcriptional roadblock) [Pubmed|32849357,22408163], in [regulon|5C398C1181CE25CAC079AFBA5871C0B72AE1AA96|GbsR regulon]
  • [protein|9F32E96AC9C32832C2F10FB125DDDC50EC9B546B|OpcR]: repression, (sterical interference with RNA polymerase access) [Pubmed|32849357,23960087], in [regulon|9F32E96AC9C32832C2F10FB125DDDC50EC9B546B|OpcR regulon]
  • [protein|6AF1CB8998BE9C71DF4F1201A7F74FF7E75CB799|RemA]: activation, [Pubmed|23646920], in [regulon|6AF1CB8998BE9C71DF4F1201A7F74FF7E75CB799|RemA regulon]

    • regulation

  • induced by choline ([protein|5C398C1181CE25CAC079AFBA5871C0B72AE1AA96|GbsR]) [Pubmed|22408163]
  • expression increases with increasing salinity [Pubmed|32849357]

    • additional information

  • [gene|6FCF6F71C5A8B2345F42BD0C3345C520FD40358B|S1290] acts as [SW|ncRNA|antisense RNA] for the [gene|3F502A4DCB1DAD7C3F968465B13C35213240515B|opuBA]-[gene|70B45E7428DB1F6AEF7D2FE3062CC86BE96674FC|opuBB]-[gene|C48798FE13B1521236F5BC5786B9A02D7BC9A336|opuBC]-[gene|1532EC3D741C5AB3D0B642741218FAE00AD460FA|opuBD] operon [PubMed|,32373088,20525796]
  • expression starts with a delay after osmotic upshift due to transcriptional interference of [SW|RNA polymerase] complexes driving synthesis of the converging opuB and [gene|6FCF6F71C5A8B2345F42BD0C3345C520FD40358B|S1290] mRNAs [pubmed|32373088]
    • view in new tab

    Biological materials

    Mutant

  • BKE33700 (Δ[gene|1532EC3D741C5AB3D0B642741218FAE00AD460FA|opuBD]::erm trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKE33700 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CACGTTCATTGACCTCTTCC, downstream forward: _UP4_TAGAATAGAAAAAGAGGCTG
  • BKK33700 (Δ[gene|1532EC3D741C5AB3D0B642741218FAE00AD460FA|opuBD]::kan trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKK33700 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CACGTTCATTGACCTCTTCC, downstream forward: _UP4_TAGAATAGAAAAAGAGGCTG

    • References

    Reviews

  • 27935846

    • Original publications

  • 10092453,9925583,10216873,21296969,20525796,22408163,23960087,23646920